Characterization of cry genes from native Bacillus thuringiensis (Bt) isolates

M. Sivaji
D. Girija

Abstract

In this study, cry genes were amplified by cry4 gene primers from the isolates of Bt-23, Bt-133, Bt-190, and Bt-242 and sequences was carried out. Different computer algorithms were used to analyze the nucleotide sequences obtained from the isolates Bt-23, Bt-133, Bt-190 and Bt-242. Homology search of nucleotide sequences obtained from isolates Bt-23, Bt-133, Bt-190, and Bt-242 with showed homology with cry4A, cry4D, cry4BLB and other dipteran specific insecticidal genes, There were five open reading frames in Bt-23cry4, with the longest one located on +3 strand starting from base 279 to 683, having a length of 405 bases. The A+T and G+C base pair composition was 60.6 per cent and 39.4 per cent in Bt-23cry4, while Bt-133cry4 A+T was 61 per cent and G+C was 38.8 per cent. Bt-190cry4 was comparatively rich in A+T (64.3%) among all sequences with very low G+C (35.7%). In contrast, Bt-242cry4 was comparatively low in A+T content (57.2%) than the other gene sequences.

How to Cite
M. Sivaji, & D. Girija. (2015). Characterization of cry genes from native Bacillus thuringiensis (Bt) isolates. Journal of Innovative Agriculture, 2(1), 39-43. Retrieved from https://www.jinagri.com/index.php/jinagri/article/view/14

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